Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse

产品说明

一般描述

Monoclonal Anti-Nitric Oxide Synthase-Brain (bNOS) (mouse IgG1 isotype) is derived from the NOS-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Nitric oxide synthase (NOS) has been localized in many different cell types. Type I NOS is found in neurons. It is a 150-160 kDa protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca²⁺- regulated NOS (cNOS). bNOS is present also in skeletal muscle, where it is complexed with dystrophin, and is absent in Duchenne′s muscular dystrophy, which perhaps accounts for symptoms of the disease.

特异性

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats.

免疫原

recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

应用

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse has been used in:

• western blot analysis
• immunocytochemistry
• double immunofluorescence
• immunofluorescence staining
• enzyme-linked immunosorbent assay (ELISA)
• dot blot immunoassay
• immunohistochemical staining

生化/生理作用

Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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