产品说明
一般描述
Monoclonal Anti-N-CAM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain. It belongs to the family of sialoglycoproteins, which arise from alternative splicing of mRNA transcribed from a single gene. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may also be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages.
Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot.
免疫原
growth cone enriched plasma membrane fraction from E17 rat forebrain.
应用
Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse has been used in:
- Immunofluorescence
- Immunohistochemistry
- Immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (3 papers)
生化/生理作用
Neural cell adhesion molecule (N-CAM) are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00164707 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | NCAM-0B11, monoclonal |
| 分子量 | antigen 140-180 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | rat, human |
| technique(s) | immunocytochemistry: suitable using cells and tissues western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | O15394 P13591 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NCAM1(4684), NCAM2(4685) rat ... Ncam1(24586), Ncam2(288280) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
