产品说明
应用
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. 35S dimethylsulfoniopropionate (DMSP) has been synthesized chemically from 35S L-methionine using LAAO from Sigma to form 35S 3-methiolpropionate.
包装
2, 5, 10 mg in glass bottle
Package size based on protein content
生化/生理作用
L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 ℃. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver.
单位定义
One Unit oxidizes one micromole of
制备说明
Dissolves in water at 1 mg/mL concentration to form a clear solution.
基本信息
| 酶学委员会(EC)编号 | 1.4.3.2 ( BRENDA | IUBMB ) |
| EC 号 | 232-564-0 |
| MDL编号 | MFCD00131454 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 类型 | Type IV |
| 形式 | aqueous suspension |
| specific activity | ≥4.0 units/mg protein |
| 分子量 | ~130 kDa |
| 包含 | toluene as preservative |
| 浓度 | ≥5.0 mg/mL |
| 溶解性 | H2O: soluble 1.0 mg/mL, clear |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H300 + H310 + H330 |
| 预防措施声明 | P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310 |
| 危险分类 | Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral |
| 储存分类代码 | 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
