产品说明
一般描述
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
特异性
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.
免疫原
Purified rat IgG
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Anti-Rat IgG (whole molecule)-FITC antibody has been used:
- in immunolabelling for confocal laser scanning microscopy (CLSM)
- in immunostaining
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.
生化/生理作用
IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
其他说明
Antibody adsorbed with human IgG.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
制备说明
Adsorbed to reduce background staining with human samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00162811 |
| NACRES | NA.46 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | FITC conjugate |
| 抗体形式 | affinity isolated antibody |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 储存条件 | protect from light |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 indirect immunofluorescence: 1:320 |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
