产品说明
生化/生理作用
Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained. It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases. NBT is also used for diagnosing microbial infections and phagocytic defects. In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.
分析说明
solubility:
250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow
基本信息
| 线性分子式 | C40H30N10O6 · 2Cl |
| 分子量 | 817.64 |
| Beilstein | 4115923 |
| MDL编号 | MFCD00012159 |
| PubChem化学物质编号 | 329770281 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 100 |
| 测定 | 90.0-110.0% (calc. on dry substance, T) |
| 杂质 | ≤10% solvent content ≤10% water |
| λ | in methanol |
| 紫外吸收 | λ: 252-262 nm Amax |
| 储存温度 | 2-8℃ |
| SMILES string | [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8 |
| InChI | 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2 |
| InChI key | FSVCQIDHPKZJSO-UHFFFAOYSA-L |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H302 - H315 - H319 - H335 |
| 预防措施声明 | P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
