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Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse

品牌
Sigma-Aldrich
货号
B6804
规格纯度
clone BAP-77, ascites fluid
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Bacterial alkaline phosphatase (BAP) is a periplasmic enzyme that is encoded by the phoA gene. The use of a BAP tag to the target gene yields a stable fusion product that does not affect the bioactivity and biodistribution of the target gene in recombinant expression vector systems. Hence, anti-BAP antibodies can be useful tools for the detection and analysis of target biomolecules fused to BAP tags.Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) (mouse IgG1 isotype) is derived from the BAP-77 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a BAP tagged fusion protein. Bacterial alkaline phosphatase (BAP) also known as PhoA, EC 3.1.3.1 is an 50 kDa protein, derived from E. coli.

免疫原

A bacterial alkaline phosphatase (BAP) tagged fusion protein.

应用

Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody is suitable for use in western blot (at 1:20,000 dilutions using purified Escherichia coli BAP) and indirect ELISA.

生化/生理作用

Bacterial alkaline phosphatase (BAP) enzyme regulates the catabolism of phosphate esters. Alkaline phosphatase appears to require export to the periplasm to show enzymatic activity. Fusions of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Fusions to positions in or near the periplasmic (extracellular) domain lead to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain give low activity.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD02095875
NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物alkaline phosphatase conjugate
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆BAP-77, monoclonal
分子量antigen ~50 kDa
species reactivitybacteria
technique(s)indirect ELISA: suitable
western blot: 1:20,000 using purified Escherichia coli BAP
同位素/亚型IgG1
运输dry ice
储存温度−20℃

Sigma-Aldrich

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