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Monoclonal Anti-β-Galactosidase antibody produced in mouse

品牌
Sigma-Aldrich
货号
G8021
规格纯度
clone GAL-13, ascites fluid
参考价格
6732.28 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

特异性

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

免疫原

β-galactosidase from E. coli

应用

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:

  • immunostaining and histochemistry
  • immunostaining and microscopy
  • immunohistochemistry
  • immunofluorescence staining
  • enzyme linked immunosorbent assay(ELISA)
  • immuno dot blot

生化/生理作用

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆GAL-13, monoclonal
包含15 mM sodium azide
species reactivitybacteria
technique(s)dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase
同位素/亚型IgG1
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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