产品说明
一般描述
Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.
Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.
免疫原
AP-2 adaptor polypeptides from bovine brain.
应用
Mouse monoclonal anti-α-adaptin antibody has been used:
- as a positive control in immunoblotting assays
- as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines
Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.
Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
生化/生理作用
Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).
外形
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00212881 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | 100/2, monoclonal |
| 包含 | 15 mM sodium azide |
| species reactivity | human, rat, bovine |
| technique(s) | western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | O95782 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... AP2A1(160) rat ... Ap2a1(308578) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
