产品说明
一般描述
Monoclonal Anti-Vitronectin (mouse IgM isotype) is derived from the VIT-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human plasma vitronectin. Vitronectin is also referred as serum-spreading factor, S-protein of complement or epibolin. This monomeric acidic protein is one of the major multifunctional cell-adhesive glycoproteins in mammalian plasma and serum. This protein is usually seen as a mixture of 75 kDa and 65 kDa polypeptides. Human plasma and serum contain 0.1-0.4 mg/ml of vitronectin which is synthesized in the liver. It is also present in amniotic fluid and urine.
特异性
By immunoblotting, the product shows no cross-reactivity with fibronectin, laminin, merosin, collagen type IV or chondroitin sulfate types A, B and C.
免疫原
human plasma vitronectin
应用
Monoclonal Anti-Vitronectin antibody produced in mouse has been used in:
- immunocytochemisry
- western blotting
- immunoprecipitation
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
生化/生理作用
Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b-9 complex. Vitronectin protects thrombin from inactivation by antithrombin III in the presence of heparin, binds and stabilizes the activity of plasminogen activator inhibitor and mediates many other physiological functions.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00162909 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | VIT-2, monoclonal |
| 分子量 | antigen 65-75 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | human |
| technique(s) | indirect ELISA: suitable indirect immunofluorescence: suitable using human cultured fibroblasts microarray: suitable western blot: 1:2,500 using a denatured and reduced preparation of purified human plasma vitronectin |
| 同位素/亚型 | IgM |
| UniProt登记号 | P04004 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... VTN(7448) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
