位置:首页 > 品牌 > Sigma-Aldrich >

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

品牌
Sigma-Aldrich
货号
H0273
规格纯度
clone IAP-28, ascites fluid
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal anti-Heat Shock Protein 25 (mouse IgG1) isotype) is derived from the IAP-28 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Heat shock (stress) proteins (HSPs) are a group of polypeptides synthesized in response to a wide variety of environmental factors, including biotic and abiotic stressors. These proteins are produced by prokaryotic and eukaryotic cells, and HSPs are among the most conserved molecules in phylogeny. Heat shock protein 25 is encoded by the gene mapped to human chromosome 7q11.23. The encoded protein belongs to the class of small HSPs. Small HSPs aggregate to form characteristic ring-shaped structures called heat shock granules. Small HSPs contains a-crystallin domain” (ACD) C-terminal domain, N-terminal domain and C-terminal extension (CTE).

特异性

The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

免疫原

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

应用

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse has been used in:

  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • radioimmunoassay (RIA)
  • immunocytochemistry
  • immunohistofluorescence assay
  • immunopurification

生化/生理作用

Small heat shock proteins (HSPs) play a vital role in stress tolerance. They act as molecular chaperones in vitro. Murine Hsp25 averts aggregation of citrate synthase and α-glucosidase. It also inhibits actin polymerization and the amyloid fibril formation of Aβ1-42. Mutations in human sHsps result in myopathies, neuropathies and cataract. The small HSPs have also been implicated in phenomena of acquired thermotolerance and neoplasia.

外形

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8 ℃. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD02189139
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆IAP-28, monoclonal
分子量antigen 25 kDa
包含15 mM sodium azide
species reactivitychicken, rabbit, rat, guinea pig, Drosophila, frog, turkey, human, mouse, bovine
technique(s)immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle
indirect ELISA: suitable
indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts
radioimmunoassay: suitable
western blot: 1:500 using turkey gizzard extract
同位素/亚型IgG1
UniProt登记号P04792
运输dry ice
储存温度−20℃
Gene Informationhuman ... HSPB1(3315)
mouse ... Hspb1(15507)
rat ... Hspb1(24471)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号