产品说明
一般描述
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
特异性
bovine IgG1 and IgG2
免疫原
Purified bovine IgG
应用
Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunohistochemistry
生化/生理作用
Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.
外形
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.46 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | alkaline phosphatase conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | secondary antibodies |
克隆 | BG-18, monoclonal |
形式 | buffered aqueous glycerol solution |
species reactivity | bovine (non-reactive to IgM) |
should not react with | goat, canine, horse, pig, sheep, human, rabbit, cat, guinea pig |
technique(s) | direct ELISA: 1:25,000 dot blot: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG western blot (chemiluminescent): 1:100,000 |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |