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Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in mouse

品牌
Sigma-Aldrich
货号
A7554
规格纯度
clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution
参考价格
4208.28 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特异性

bovine IgG1 and IgG2

免疫原

Purified bovine IgG

应用

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry

生化/生理作用

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

外形

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物alkaline phosphatase conjugate
抗体形式purified immunoglobulin
antibody product typesecondary antibodies
克隆BG-18, monoclonal
形式buffered aqueous glycerol solution
species reactivitybovine (non-reactive to IgM)
should not react with goat, canine, horse, pig, sheep, human, rabbit, cat, guinea pig
technique(s)direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000
同位素/亚型IgG1
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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