产品说明
一般描述
Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.
特异性
该抗体对人 IgG 的 Fc 部分具有特异性，并识别所有人 IgG 亚类共有的表位。在 IUIS/WHO 研究中，该抗体被用作 Fc 特异性试剂。
应用
Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in:
- Fluorescent Dot Immunobinding Assay (F-DIBA)
- Particle Immunofluorescent Assay (F-IFMA)
- flow cytometry
生化/生理作用
Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding.
外形
Solution in 0.01 M phosphate buffer pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | FITC conjugate |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| 克隆 | HP-6017, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | sheep, horse (IgG), rabbit, goat, human |
| 储存条件 | protect from light |
| technique(s) | dot immunobinding: 1:16 particle immunofluorescence: 1:16 |
| 同位素/亚型 | IgG2a |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H317 - H334 |
| 预防措施声明 | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| 危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
