产品说明
一般描述
Catenin are distinct peripheral cytosolic proteins, α, β, and γ -catenin (102 kDa, 94 kDa and 86 kDa, respectively) are found in varying abundance in many developing and adult tissues. Within its conserved regions, α-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions.
特异性
Does not cross-react with β-catenin or γ-catenin (plakoglobin).
免疫原
synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse α-catenin conjugated to KLH.
应用
Anti-α-Catenin antibody has been used:
- in dot blot immunoassay
- in immunoblotting
- in immunofluorescence
- in western blotting
- in immunoprecipitation
- in immunofluorescence staining
- in coimmunoprecipitation
生化/生理作用
Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis. Catenins link E-cadherin to other integral membrane proteins such as Na+ /K+ -ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, sarcoma (Src) and Yes kinases and are modulated by Wnt-1 protooncogene. They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. α-Catenin appears to be capable of interacting with N-cadherin and P-cadherin. Absence of α-catenin is found in certain tumor cell lines. Frequent reduction of α-catenin levels in human carcinomas of the esophagus, stomach and colon is also reported. Prostate cancer development appears to be correlated with α-catenin gene deletions.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | whole antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 分子量 | antigen ~102 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | several mammalian species |
| technique(s) | dot blot: suitable using α-catenin peptide amino acids 890-901 conjugated to BSA immunohistochemistry (frozen sections): 1:2,000 using bovine kidney sections indirect immunofluorescence: 1:2,000 using cultured MDBK cells microarray: suitable western blot: 1:4,000 using cultured MDBK cells extract |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CTNNA1(1495) mouse ... Ctnna1(12385) rat ... Ctnna1(307505) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
