产品说明
一般描述
Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.
Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.
特异性
Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.
免疫原
Purified rat IgG
应用
Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- slot blot immunoassay
- immunoblotting
- dot blot
- immunocytochemistry
生化/生理作用
Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.
外形
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
储存及稳定性
For continuous use, store at 0-5 ℃. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00162826 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | secondary antibodies |
| 克隆 | R2A-2, monoclonal |
| 包含 | 15 mM sodium azide |
| technique(s) | capture ELISA: suitable dot blot: suitable immunocytochemistry: suitable indirect ELISA: 1:5,000 western blot: suitable |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
