产品说明
一般描述
ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas.
Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO.
免疫原
recombinant, human ARNO (ARFnucleotide-binding-site opener).
应用
To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO.
Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD03454755 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CYT2-21, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 40 kDa |
| species reactivity | rat, human |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 5-10 μg/mL using a whole extract of rat brain |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q99418 |
| 储存温度 | −20℃ |
| Gene Information | human ... CYTH2(9266) rat ... Cyth2(116692) |
