产品说明
一般描述
Plasmid pAR1219 is a pBR322-based vector that expresses T7 RNA Polymerase under control of the IPTG inducible lacUV5 promoter and is intended for use with the TargeTron Gene Knockout System (TA0100).
应用
Many TargeTron system plasmids use the T7 promoter for intron expression. By co-transforming plasmid pAR1219 with the TargeTron pACD4 plasmids, the T7 promoter can be used to express the intron and disrupt chromosomal genes in alternative hosts such as Salmonella typhimurium and Shigella flexneri. Chromosomal gene disruptions in non-DE3 strains of E. coli can also be performed using pAR1219 with the pACD4 intron expression plasmids. TargeTron™ Vector pAR1219 has been used for the overexpression of T7 RNA polymerase in the preparation of S30-T7 lysate. It has also been used to transform MRE600 E. coli strains.
包装
2 μg in poly bottle
制备说明
To use pAR1219 in conjunction with the pACD4 plasmids, simply co-transform both plasmids and select in a liquid medium containing: 50 mg/ml ampicillin, 25 mg/ml chloramphenicol, and 1% glucose. Glucose is typically included to provide additional suppression of the lac UV5 promoter prior to IPTG-induction.
其他说明
For more information and to view applications data, please visit www.sigma-aldrich.com/targetron.
法律信息
TargeTron is a trademark of InGex, LLC
产品性质
质量水平 | 200 |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |