产品说明
一般描述
Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.
特异性
Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.
免疫原
synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).
应用
Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:
- in immunoblotting and dot blot
- in immunoprecipitation
- in immunohistochemistry
- in enzyme linked immunosorbent assay (ELISA)
- in western blotting
- in chromatin immunoprecipitation(ChiP)
生化/生理作用
Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
外形
Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02096544 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 55 kDa |
| species reactivity | mouse, human |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells microarray: suitable western blot: 1:20,000 using nuclear extract from human HeLa cells |
| UniProt登记号 | Q92769 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HDAC2(3066) mouse ... Hdac2(15182) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
