产品介绍：
产品说明
应用
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
生化/生理作用
BS-I对末端α-
其他说明
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-
单位定义
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 ℃.
外形
Contains sodium citrate buffer salts and calcium chloride
制备说明
Prepared from peroxidase type VI using a modification of the method of O′Sullivan, et al. Repurified by affinity chromatography after conjugation.
分析说明
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水(pH6.8)对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
基本信息
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | Bandieraea simplicifolia |
| 偶联物 | peroxidase conjugate |
| 测定 | ≥90% protein basis (Warburg-Christian) |
| 形式 | lyophilized powder |
| 效能 | <200 μg per mL agglutination activity (using human blood group B erythrocytes) |
| peroxidase activity | 40-160 units/mg protein |
| 组成 | Protein, ~95% modified Warburg-Christian |
| technique(s) | blood typing: suitable fractionation: suitable |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
