产品说明
一般描述
Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.
The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.
特异性
Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.
免疫原
synthetic connexin-43 peptide (362-381).
应用
Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:
- fluorescent immunostaining
- western blotting
- immunocytochemistry
- preparation of immuno-affinity columns
生化/生理作用
Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00803532 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | CXN-6, monoclonal |
| 分子量 | antigen 43 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | rat, human, hamster, bovine, mouse |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunohistochemistry (frozen sections): suitable indirect ELISA: suitable microarray: suitable western blot: 1:8,000-1:16.000 using mouse whole brain extract |
| 同位素/亚型 | IgM |
| UniProt登记号 | P17302 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... GJA1(2697), GJA1P1(2698) mouse ... Gja1(14609) rat ... Gja1(24392) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
