产品说明
一般描述
Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes.
特异性
Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2.
应用
Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:
- co-immunoprecipitation
- chromatin immunoprecipitation (ChIP)
- western blot
- immunohistochemistry
- enzyme linked immunosorbent assays (ELISA)
包装
200 μL in glass insert
生化/生理作用
Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02096544 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | HDAC2-62, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~55 kDa |
| species reactivity | rat, canine, human, chicken, mouse, bovine |
| 包装 | antibody small pack of 25 μL |
| technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.25-0.5 μg/mL using total cell extracts from NIH3T3 cells |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | Q92769 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HDAC2(3066) mouse ... Hdac2(15182) rat ... Hdac2(84577) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
