位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Synapsin I Antibody

品牌
Sigma-Aldrich
货号
AB1543P
规格纯度
Chemicon®, from rabbit
参考价格
5529.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are encoded by three different genes, synapsin I, II and III, and different neuron terminals will encode different amounts of these; synapsin will make up 1% of total brain protein at any one time.
Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter.

特异性

Synapsin I (Mr of 80,000 and 77,000). Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Immunolabeling is blocked by preadsorption of the antibody with synapsin I.

免疫原

Synapsin I (mixture of Ia & Ib) purified from bovine brain.

应用

Research Sub Category
Synapse & Synaptic Biology
This Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I.
Research Category
Neuroscience
Western blotting:
1:200-1:1,000 dilution of a previous lot was used.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein.
Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Immunohistochemistry:
1:500-1:2,500
ELISA:
1:2,500-1:10,00
Optimal working dilutions must be determined by the end user.

质量

Immunohistochemistry(paraffin):
Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval:
Rat Hippocampus

目标描述

77 & 80 kDa

联系

Replaces: MAB355

外形

Purified rabbit polyclonal in buffer lyophilized from 5 mM ammonium bicarbonate. Reconstitute with 50 μL of PBS.
ImmunoAffinity Purified
Format: Purified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Brain tissue.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
species reactivitymouse, bovine, rat, human
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NM_006950.3
UniProt登记号P17600
运输dry ice
Gene Informationhuman ... SYN1(6853)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号