产品说明
一般描述
Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).
Neurocan is a proteoglycan which is expressed in the nervous tissue.
免疫原
embryonic rat brain proteoglycans.
应用
Anti-Neurocan antibody, Mouse monoclonal has been used in:
- western blotting
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
- immunocytochemistry
- immunohistochemistry
生化/生理作用
Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.
Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.
目标描述
Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02263808 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 650.24, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 260 kDa (additional band at 160 kDa) |
| species reactivity | rat |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~1 mg/mL |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (frozen sections): suitable indirect ELISA: suitable western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P55067 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | rat ... Ncan(58982) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
