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Anti-Brain Derived Neurotrophic Factor Antibody

品牌
Sigma-Aldrich
货号
AB1513
规格纯度
serum, Chemicon®
参考价格
5426.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.
The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.

免疫原

Recombinant Human Brain Derived Neurotrophic Factor (BDNF).

应用

Immunohistochemistry: 1:200-1:2,000 (see suggested protocol).
Immunoblotting: 1:200-1:2,000 we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochemistry 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH 7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C.
ELISA: 1:200-1:2,000
Inhibition of biological activity in vitro: 1:10-1:50
Use neat for in vivo animal studies.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, WB, IH.
Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain

外形

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any insoluble material.

储存及稳定性

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源sheep
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, rat
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_001709.3
NM_170731.3
NM_170732.3
NM_170733.2
NM_170734.2
NM_170735.4
UniProt登记号P23560
运输wet ice
Gene Informationhuman ... BDNF(627)

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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