产品说明
一般描述
PAR-4 is a pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells and sensitizing the cells to diverse apoptotic stimuli causing tumor regression. PAR-4 also seems to be a transcriptional repressor and is involved in regulating APP expression and the cleavage activity of BACE1. PAR-4 interacts with several proteins including WT1, but also various kinases, and structural proteins like Actin, BACE1, SPSB1, SPSB2, and SPSB4. PAR4 is localized in the cytoplasm in non-apoptotic cells but is nuclear localized in cancer and apoptotic cells. PAR-4 is widely expressed and elevated in neurodegenerative diseases such as ALS, Alzheimer, Parkinson and Huntington diseases as well as stroke. PAR-4 is down regulated in cancers.
免疫原
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human PAR-4.
应用
Research Sub Category
Apoptosis - Additional
Anti-PAR-4 Antibody is an antibody against PAR-4 for use in Western Blotting.
Research Category
Apoptosis & Cancer
质量
Evaluated by Western Blotting in Y-79 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected PAR-4 in 10 µg of Y-79 cell lysate.
目标描述
~45 kDa observed. The calculated molecular weight is 36 kDa, however PAR-4 has been shown as a ~45 kDa band in some western blots (Masters, S.L., et al. (2006). Nature Structural & Mole. Biol. 13(1):77-84).
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI登记号 | NP_002574 |
| UniProt登记号 | Q96IZ0 |
| 运输 | wet ice |
| Gene Information | human ... PAWR(5074) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
