产品说明
一般描述
REDExtract-N-Amp™ PCR反应混合物是包含缓冲液、盐、dNTP和REDTaq DNA聚合酶的PCR预混液。
该PCR ReadyMix要与Sigma的Extract-N-Amp植物PCR试剂盒以及Extract-N-Amp组织PCR试剂盒一起使用。所有Extract-N-Amp试剂盒均可为每次提取进行一次PCR反应。然而，如果需要更多的PCR反应，则可能需要补充额外的PCR ReadyMix。REDExtract-N-Amp PCR ReadyMix中含有一种惰性染料，可作为示踪染料以实现方便地将PCR产物加载到琼脂糖凝胶上进行分析。
应用
REDExtract-N-Amp™ PCR Master Mix has been used:
- for the genotyping of fixed zebrafish embryo samples
- in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp™ Plant PCR Kit
- in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp™ seed PCR kit.
REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.
特点和优势
- A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
- Convenience- Hot-start feature in JumpStart™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
- Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
- High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.
法律信息
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
JumpStart is a trademark of sigma-aldrich Co. LLC
REDExtract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.55 |
产品性质
| 质量水平 | 200 |
| 用途 | sufficient for 100 reactions 1.2 mL sufficient for 100 amplifications sufficient for 1000 reactions 12 mL sufficient for 1000 amplifications sufficient for 10000 reactions 125 mL sufficient for 10000 amplifications |
| 特点 | dNTPs included hotstart |
| 颜色 | red |
| application(s) | agriculture |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
