产品说明
应用
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
生化/生理作用
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 ℃.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
单位定义
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 ℃ using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).
外形
Partially purified powder.
基本信息
| 酶学委员会(EC)编号 | 3.4.21.50 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130333 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 形式 | partially purified powder |
| specific activity | ≥20,000 units/mg solid |
| 分子量 | 27 kDa |
| 异质活性 | Collagenase, present |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H334 |
| 预防措施声明 | P261 - P342 + P311 |
| 危险分类 | Resp. Sens. 1 |
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
