产品说明
应用
据报道,来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸
比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性的影响,导致 6-MAM 转化为吗啡
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.
特点和优势
Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 ℃.
单位定义
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 ℃ at pH 3.8 (30 min assay).
外形
Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.
基本信息
酶学委员会(EC)编号 | 3.2.1.31 ( BRENDA | IUBMB ) |
MDL编号 | MFCD00130629 |
产品性质
质量水平 | 200 |
重组 | expressed in proprietary host |
形式 | aqueous solution |
浓度 | 300000-400000 units/mL |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |