产品说明
一般描述
α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.
应用
α-Actinin from chicken gizzard has been used:
- as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
- in in vitro motility experiments to test its effect on actin filament movement
- to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies
包装
5 mg in poly bottle
Package size based on protein content
生化/生理作用
α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.
外形
Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride
制备说明
Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.
Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).
基本信息
MDL编号 | MFCD00130473 |
NACRES | NA.32 |
产品性质
生物来源 | chicken gizzard |
质量水平 | 200 |
测定 | ~80% α-actinin basis (SDS-PAGE) |
形式 | ammonium sulfate suspension |
分子量 | 100 kDa |
UniProt登记号 | P05094 P12814 P20111 Q7TPR4 Q90734 Q9Z1P2 |
储存温度 | 2-8℃ |
Gene Information | chicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918) human ... ACTN1(87) mouse ... ACTN1(109711) rat ... ACTN1(81634) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |