位置:首页 > 品牌 > Sigma-Aldrich >

α-Actinin from chicken gizzard

品牌
Sigma-Aldrich
CAS
11003-00-2
货号
A9776
规格纯度
~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension
参考价格
1967.39 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.

应用

α-Actinin from chicken gizzard has been used:

  • as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
  • in in vitro motility experiments to test its effect on actin filament movement
  • to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies

包装

5 mg in poly bottle
Package size based on protein content

生化/生理作用

α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.

外形

Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

制备说明

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.
Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

基本信息

MDL编号MFCD00130473
NACRESNA.32

产品性质

生物来源chicken gizzard
质量水平200
测定~80% α-actinin basis (SDS-PAGE)
形式ammonium sulfate suspension
分子量 100 kDa
UniProt登记号P05094
P12814
P20111
Q7TPR4
Q90734
Q9Z1P2
储存温度2-8℃
Gene Informationchicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918)
human ... ACTN1(87)
mouse ... ACTN1(109711)
rat ... ACTN1(81634)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号