产品说明
一般描述
Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.
Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins.
特异性
Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.
免疫原
neurofilaments from pig spinal cord.
应用
Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Monoclonal anti-neurofilament 160 antibody has been used in
- Immunoblotting.
- Immunohistochemistry
- Immunofluorescence
生化/生理作用
Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01322393 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | NN18, monoclonal |
| 分子量 | antigen 160 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | pig, human |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections immunohistochemistry (frozen sections): suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P07197 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | chicken ... NEFM(396206) human ... NEFM(4741) mouse ... Nefm(18040) rat ... Nefm(24588) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
