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α-Glycerophosphate Dehydrogenase from rabbit muscle

α-甘油磷酸脱氢酶 来源于兔肌肉

品牌
Sigma-Aldrich
CAS
9075-65-4
货号
G6751
规格纯度
Type I, ammonium sulfate suspension, 100-300 units/mg protein
参考价格
727.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

应用

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

  • in the reaction mixture to measure the glycerol kinase activity
  • to demonstrate compartmentalized enzymatic reactions where NADH is involved
  • in the reaction mixture assay for L-fuculose-1-phosphate aldolase

生化/生理作用

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

单位定义

在 pH 7.4,25℃ 下,一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。

外形

溶于 3.2M(NH4)2SO4 和 0.1g/L EDTA 溶液中的结晶悬浮液,pH 6.0

制备说明

根据改良的 Fondy 法进行制备。

分析说明

缩二脲法测定蛋白

基本信息

酶学委员会(EC)编号1.1.1.8 ( BRENDA | IUBMB )
EC 号232-982-3
MDL编号MFCD00130487
NACRESNA.54

产品性质

质量水平200
类型Type I
形式ammonium sulfate suspension
specific activity100-300 units/mg protein
异质活性Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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