产品介绍：
产品说明
细胞系来源
Human Caucasian colon carcinoma
细胞系描述
Subclone of parent HT-29 which was established from an adenocarcinoma from a 44 year old Caucasian female. Tumorigenic in nude mice and has ability to grow in agar.
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | human colon |
| 生长模式 | Adherent |
| 核型 | 2n = 46, hypertriploid |
| 形态学 | Epithelial |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
