产品介绍：
产品说明
细胞系来源
Human ileocecal adenocarcinoma
细胞系描述
From a 67 year old male. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
应用
Tumourigenicity studies
培养基
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | human intestine |
| 生长模式 | Adherent |
| 核型 | 2n = 46, pseudodiploid |
| 形态学 | Epithelial |
| 产品 | Carcinoembryonic antigen (CEA) |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
