C75

产品说明

细胞系来源

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage D

细胞系描述

The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes′ stage D

DNA图谱分析

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17

培养基

Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm^² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 ℃. C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质