WRL 68 (HeLa derivative)

产品说明

细胞系来源

Human cervix carcinoma

细胞系描述

The human hepatic cell line WRL-68 exhibits a morphology similar to hepatocytes and hepatic primary cultures. Cells have been shown to secrete albumin and α-feto protein and express liver specific enzymes such as alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase and alkaline phosphatase. Previously contaminated with Mycoplasma; treated and cured at ECACC. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

In vitro hepatic model

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37℃.

其他说明

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产品性质