产品说明
一般描述
Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.
应用
Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.
包装
25 g in glass bottle
5 g in poly bottle
质量
材料可以是一溴一氯和二氯 ZnCl2 盐的混合物。标签列出了卤素含量。
分析说明
基于一溴一氯 ZnCl2 盐分子量 (653.2)的染料含量。
基本信息
| 线性分子式 | C27H35BrClN3 · ZnCl2 |
| 分子量 | 653.24 |
| 颜色索引号 | 42590 |
| Beilstein | 3914106 |
| EC 号 | 230-415-4 |
| MDL编号 | MFCD00151094 |
| PubChem化学物质编号 | 24897293 |
| NACRES | NA.47 |
产品性质
| 质量水平 | 200 |
| 测定 | ~85% |
| 形式 | powder |
| 组成 | Dye Content, ≥83% |
| pH范围 | 0.2 - 1.8, yellow to blue |
| mp | >300 ℃ (lit.) |
| 溶解性 | water: 1 mg/mL |
| εmax | >11100.00 at 417-425 nm in water at 0.005 g/L >16000 at 311-319 nm in water at 0.005 g/L >60000 at 626-634 nm in water at 0.005 g/L |
| application(s) | diagnostic assay manufacturing hematology histology |
| 储存温度 | room temp |
| SMILES string | [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C |
| InChI | 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4 |
| InChI key | RRKSQWOQZFIQKW-UHFFFAOYSA-J |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H314 - H335 - H411 |
| 预防措施声明 | P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
| 危险分类 | Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Gloves |
