产品说明
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
培养基
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | human colon |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Epithelial |
产品 | Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |