HeLa S3

产品说明

细胞系来源

Human cervix carcinoma

细胞系描述

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

应用

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37℃. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

其他说明

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产品性质