产品说明
细胞系来源
Human colon adenocarcinoma
细胞系描述
Colorectal adenocarcinoma which is tumorigenic in nude mice.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
应用
HCT-15 cell line has been used to study the role and efficiency of nexrutine (NX), an extract from Phellodendron amurense, against colon cancer.
培养基
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS) (heat inactivated).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | human colon |
生长模式 | Adherent |
核型 | 2n = 46, quasidiploid |
形态学 | Epithelial-like |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |