位置:首页 > 品牌 > Sigma-Aldrich >

143B

货号
CB_91112502
规格纯度
91112502
参考价格
4474.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

细胞系来源

Human bone osteosarcoma TK-

细胞系描述

Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (Sigma Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+.

应用

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

培养基

EMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源human bone
生长模式Adherent
核型Not specified
形态学Not specified
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
相关疾病cancer
运输dry ice
储存温度−196℃

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号