产品说明
应用
α2-Macroglobulin was used to study impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-β which is an early biomarker for mild cognitive impairment preceding Alzheimer′s disease. It was also used in the control of the classical and the MBL pathway of complement activation.
通过与蛋白酶形成 1:1 复合物抑制各种内切蛋白酶。当蛋白酶裂解巨球蛋白"诱饵"序列时，巨球蛋白将重排并捕获蛋白酶。
生化/生理作用
α2-Macroglobulin is found abundantly in plasma and interstitial fluids. The protease-α2-M balance plays an important role in mediating inflammatory tissue destruction. Serum levels of α2-M and protease-α2-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis, and other inflammatory diseases, and oxidant inactivation of α2-M may contribute to tissue destruction during inflammation.
α2-Macroglobulin (α2M) is a multifunctional protein that is a broad spectrum protease inhibitor. α2M is a large homotetrameric glycoprotein (720 kDa) that is connected by disulfide-linked dimers which non-covalently interact to give the quaternary structure. α2M is found in normal plasma at a concentration of 220-230 mg/dl accounting for 3-5% of the total plasma protein.
包装
根据蛋白质含量确定包装尺寸
外形
由 0.02M Tris、0.13M 的甘氨酸 (pH 8.0) 以及 0.08M 海藻糖冻干制得。
分析说明
经检测，每位捐献者的血浆 HIV-1/HIV-2 抗体、HCV 和 HbSAg 抗体均为阴性。
基本信息
| MDL编号 | MFCD00130472 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human plasma |
| 产品线 | BioUltra |
| 测定 | ≥98% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | ~720 kDa (four glycoprotein subunits) |
| 组成 | Protein, 15-30% biuret |
| technique(s) | inhibition assay: suitable |
| 溶解性 | water: soluble 10 mg protein/mL, clear, colorless |
| UniProt登记号 | P01023 |
| 储存温度 | −20℃ |
| Gene Information | human ... A2M(2) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
