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Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse

品牌
Sigma-Aldrich
货号
M7431
规格纯度
clone 1B3B9, purified immunoglobulin, buffered aqueous solution
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAP Kinase 2 recognizes the 42 kDa MAPK2 encoded by the mapk gene. The antibody may weakly react with MAPK1 (ERK1) and only binds denatured MAP kinase.

免疫原

recombinant mouse MAP kinase.

应用

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody is suitable for detection by immunoblotting, at a working concentration of 0.5 μg/mL, using whole cell lysates of human A431, mouse 3T3 fibroblasts or rat L6 cells. Detection of MAPK2 (ERK-2) was possible in human colon adenocarcinoma cells, HT-29. Immunoprecipitation applications in mouse 3T3 fibroblasts require a concentration of 4 μg. The antibody may be suitable for protein microarray.

外形

Solution in phosphate buffered saline containing 0.05% sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01633614
NACRESNA.44

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆1B3B9, monoclonal
形式buffered aqueous solution
分子量antigen 42 kDa
species reactivityhuman, rat, mouse
technique(s)immunoprecipitation (IP): 4 μg using denatured ERK-2 from a mouse 3T3 fibroblast lysate
microarray: suitable
western blot: 0.5 μg/mL using lysates from human A431 cells, mouse 3T3 fibroblasts or rat L6 cells
同位素/亚型IgG2a
UniProt登记号P28482
运输dry ice
储存温度−20℃
Gene Informationhuman ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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