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Anti-Sheep IgG (whole molecule)–Alkaline Phosphatase antibody produced in donkey

品牌
Sigma-Aldrich
货号
A5187
规格纯度
affinity isolated antibody, buffered aqueous glycerol solution
参考价格
2200.31 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Affinity isolated antibody is obtained from anti-sheep IgG antiserum by immunospecific purification which removes essentially all donkey serum proteins, including immunoglobulins, which do not specifically bind to sheep IgG. Anti-Sheep IgG is conjugated to alkaline phosphatase by protein cross-linking with 0.2% glutaraldehyde. Specificity of the antiserum is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, the antiserum reacts specifically with normal sheep serum and sheep IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the product followed by diffusion against anti-horse IgG and anti-donkey whole serum results in single arcs of precipitation.
IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody binds to all sheep immunoglobulins.

特异性

normal sheep serum and sheep IgG

免疫原

Purified sheep IgG

应用

Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
The anibody may be used in applications such as immunoblotting, dot blot and direct ELISA at a dilution of 1:30,000
Solid-Phase Decapeptide Assays were performed using alkaline phosphatase conjugated donkey anti-sheep IgG at 1:1000 for 2-3 hours at room temperature.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 50% glycerol, 1% bovine serum albumin, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162844
NACRESNA.46

产品性质

质量水平200
生物来源donkey
偶联物alkaline phosphatase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous glycerol solution
technique(s)direct ELISA: 1:30,000
western blot: 1:30,000
运输wet ice
储存温度2-8℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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