产品说明
一般描述
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Purified human IgA, IgG and IgM are used to develop anti-sera in rabbits. The IgG fraction of each anti-sera is purified and conjugated to Fluorescein Isothiocyanate (FITC), in an alkaline reaction. Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody specifically recognizes human IgA, IgG and IgM.
免疫原
Purified human IgA, IgG, and IgM
应用
Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a working antibody dilution of 1:128. For anti-nuclear antibody assay of acetone-fixed rat liver cells, a minimum dilution of 1:128 is recommended. For direct labeling of human tonsil sections, a dilution of 1:80 may be used. This antibody was used to stain C2C12 cell line derived from mouse myoblasts at a dilution of 1:100
Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit has been used in immunohistochemical analysis and immunofluorescence
生化/生理作用
IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00239694 |
| NACRES | NA.46 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | FITC conjugate |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 储存条件 | protect from light |
| technique(s) | ANA-indirect immunofluorescence: 1:128 direct immunofluorescence: 1:80 |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
