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Anti-Human IgG (Fab specific)-Alkaline Phosphatase antibody produced in goat

抗 人 IgG(Fab 特异性)-碱性磷酸酶 山羊抗

品牌
Sigma-Aldrich
货号
A8542
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
1713.33 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The conjugate is specific for human IgG and human IgG Fab fragment. The conjugate shows no reactivity with human IgG Fc fragment, mouse IgG or rat IgG.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Antiserum is developed in goat using purified human IgG Fab fragment as the immunogen. Antibody is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Goat anti-human IgG is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

特异性

Binds all human Igs.
Provides reduced background staining with mouse or rat samples.

免疫原

Developed in goat using purified human IgG Fab fragment

应用

Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and chemiluminescent ELISA.
Nuclear proteins isolated from primary neuronal cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-human Fab specific as the secondary antibody.

生化/生理作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

其他说明

No cross-reaction with mouse and rat IgG.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162432
NACRESNA.46

产品性质

质量水平300
生物来源goat
偶联物alkaline phosphatase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
technique(s)direct ELISA: 1:40,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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