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Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep

抗-小鼠IgG(全分子)–过氧化物酶 绵羊抗

品牌
Sigma-Aldrich
货号
A5906
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
2634.11 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody reacts with mouse IgG in vitro and in mouse serum and biological fluids. It does not react with human serum proteins.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgG.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu Red into detectable chromophores, light-emitters or fluorescers, respectively.

免疫原

Purified mouse IgG

应用

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in peptide arrays (1:500) and western blot. The product can also be used for direct ELISA (1:10,000).
Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. Immunostaining was performed using HRP-conjugated sheep anti-mouse IgG (SAM) diluted in 1% BSA-TBS. HRP was visualized using Na nitroprusside and o-clianisidine (Sigma).

包装

1 mL in glass bottle

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

制备说明

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

法律信息

Ampliflu is a trademark of sigma-aldrich Co. LLC

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162644
NACRESNA.46

产品性质

质量水平200
生物来源sheep
偶联物peroxidase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
technique(s)direct ELISA: 1:10,000
运输dry ice
储存温度−20℃

安全信息

象形图GHS07GHS09
警示用语:Warning
危险声明H315 - H317 - H319 - H411
预防措施声明P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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