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Terrific Broth

品牌
Sigma-Aldrich
货号
T9179
规格纯度
EZMixTM powder microbial growth medium
参考价格
1035.33 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

应用

Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:

  • as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
  • to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
  • Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)

包装

1 kg in poly bottle

特点和优势

Terrific Broth EZMix powder provides:

  • Granulated, dust-free format for safer handling and faster mixing
  • A budget-friendly alternative to liquid
  • Modified formulation (without glycerol)
  • Easy scale-up using larger package sizes

组分

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4

重悬

Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 ℃ to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

法律信息

EZMix is a trademark of Merck KGaA, Darmstadt, Germany

基本信息

NACRESNA.85

产品性质

质量水平200
等级for molecular biology
描述TB; quick dissolve, dust-free formulation
无菌性non-sterile
形式powder
application(s)agriculture
food and beverages
储存温度room temp
适用性nonselective for Escherichia coli
nonselective for coliforms

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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