V79-4

产品说明

细胞系来源

Hamster Chinese lung

细胞系描述

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

应用

Mutagenesis and mammalian cell genetic studies

培养基

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37℃ using 0.25% trypsin/EDTA.

其他说明

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产品性质