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A9(Hamprecht)

货号
CB_08062526
规格纯度
08062526
参考价格
4633 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

细胞系来源

Mouse Fibroblast (L cells)

细胞系描述

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

应用

Used in the generation of cell hybrids.

培养基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37℃.

其他说明

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产品性质

生物来源mouse connective tissue
生长模式Adherent
核型Modal No of chromosomes is 54
形态学Fibroblast
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

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