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3T3-F442A

货号
CB_00070654
规格纯度
00070654
参考价格
4633 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

细胞系来源

Mouse pre-adipocytes

细胞系描述

Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 h.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.

应用

Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.

培养基

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% Newborn Calf Serum(NBCS) (N4637)

传代培养常规

Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37℃. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use New

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源mouse adipose tissue
生长模式Adherent
核型Not specified
形态学Fibroblast-like
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

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