产品说明
一般描述
Product has lost contact inhibition
细胞系来源
Mouse Swiss Albino embryo fibroblast
细胞系描述
Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.
应用
Contact inhibition and virus studies: polyoma and SV40 viruses
培养基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
| 生物来源 | mouse embryo |
| 生长模式 | Adherent |
| 核型 | 2n = 40; hypertriploid |
| 形态学 | Fibroblast |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
